Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improving yeast two-hybrid screening systems.

Manfred Koegl1, Peter Uetz

  • 1Translational Research, and Genomics and Proteomics Core Facilities, German Cancer Research Center, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany. m.koegl@dkfz.de

Briefings in Functional Genomics & Proteomics
|January 26, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The protein interactome of Escherichia coli carbohydrate metabolism.

PloS one·2025
Same author

An inclusive Research Education Community (iREC) Model to Facilitate Undergraduate Science Education Reform.

Frontiers in education·2024
Same author

Targeting cancer with small-molecule pan-KRAS degraders.

Science (New York, N.Y.)·2024
Same author

Chasing Red Herrings: Palladium Metal Salt Impurities Feigning KRAS Activity in Biochemical Assays.

Journal of medicinal chemistry·2024
Same author

Confounding Factors in Targeted Degradation of Short-Lived Proteins.

ACS chemical biology·2024
Same author

A globally integrated structure of taxonomy to support biodiversity science and conservation.

Trends in ecology & evolution·2023
Same journal

From consensus structure prediction to RNA gene finding.

Briefings in functional genomics & proteomics·2009
Same journal

The use of covariance models to annotate RNAs in whole genomes.

Briefings in functional genomics & proteomics·2009
Same journal

Annotating non-coding transcription using functional genomics strategies.

Briefings in functional genomics & proteomics·2009
Same journal

Establishing legitimacy and function in the new transcriptome.

Briefings in functional genomics & proteomics·2009
Same journal

The role of RNA molecules in cellular biology. Editorial.

Briefings in functional genomics & proteomics·2009
Same journal

Sequencing genomes: from individuals to populations.

Briefings in functional genomics & proteomics·2009
See all related articles

Yeast two-hybrid screening effectively detects protein-protein interactions, enabling proteome-wide mapping. However, limitations necessitate complementary high-throughput methods for comprehensive interaction discovery.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Proteomics

Background:

  • Protein-protein interactions (PPIs) are fundamental to cellular processes.
  • Yeast two-hybrid (Y2H) is a key method for detecting PPIs.
  • High-throughput screening is crucial for mapping PPIs across entire proteomes.

Purpose of the Study:

  • To review and compare high-throughput adaptations of Y2H screening.
  • To discuss the limitations of Y2H for proteome-wide interaction mapping.
  • To explore alternative methods for complementing PPI discovery.

Main Methods:

  • Review of literature on Yeast Two-Hybrid (Y2H) screening.
  • Comparison of different high-throughput Y2H methodologies.
  • Analysis of alternative high-throughput protein interaction detection techniques.

Related Experiment Videos

Main Results:

  • Y2H screening has been optimized for high-throughput, enabling systematic PPI mapping.
  • Y2H screens alone are insufficient for comprehensive proteome-wide interaction mapping.
  • Alternative and complementary high-throughput approaches are essential.

Conclusions:

  • Optimized Y2H methods facilitate large-scale PPI studies.
  • Limitations of Y2H necessitate the use of parallel high-throughput strategies.
  • A combination of methods is required for complete organism-wide PPI mapping.