Metabolic flux analysis in biotechnology processes
Related Experiment Video
Updated: Feb 1, 2026

Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis
Published on: February 3, 2022
Shintaro Iwatani1, Yohei Yamada, Yoshihiro Usuda
1Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc, Kawasaki-shi, Kanagawa, 210-8681, Japan. shintaro_iwatani@ajinomoto.com
Abstract:
Metabolic flux analysis (MFA) has become a fundamental tool of metabolic engineering to elucidate the metabolic state of the cell and has been applied to various biotechnological processes. In recent years, considerable technical advances have been made. Developments of analytical instruments allow us to determine (13)C labeling distribution of intracellular metabolites with high accuracy and sensitivity. Moreover, kinetic information of intracellular label distribution during isotopic instationary enables us to calculate metabolic fluxes with shortened experimental time and decreased amount of labeled substrate. The (13)C MFA may be one of the most promising approaches for the target estimation to improve strain performances and production processes.
More Related Videos
Related Concept Videos
Plant Breeding and Biotechnology
What is Metabolism?
Electric Flux
Magnetic Flux
Suppose a surface is divided into elements of area dA. For each element, the component of the magnetic field that is normal to the...
Calculation of Electric Flux
Carbohydrate Metabolism
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...

