Related Experiment Video
Updated: Jul 7, 2026
14:04
Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
Published on: February 1, 2010
Interaction trap/two-hybrid system to identify interacting proteins.
E A Golemis1, I Serebriiskii, R L Finley
1Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA.
Current Protocols in Cell Biology
|January 30, 2008
Summary
This study introduces a novel yeast-based system for identifying interacting proteins. This method utilizes transcriptional activation of a reporter system to detect protein complexes and interactions.
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- Understanding protein-protein interactions is crucial for elucidating cellular mechanisms.
- Existing methods for detecting protein interactions can be complex and time-consuming.
Purpose of the Study:
- To present a straightforward protocol for identifying interacting proteins.
- To establish a yeast-based system for detecting protein complex formation.
Main Methods:
- Utilizing yeast as a biological system.
- Employing transcriptional activation of a reporter system.
- Testing for protein complex formation based on expected interactions.
Main Results:
- Demonstrated a functional system for detecting protein interactions in yeast.
- Successfully identified interacting proteins using the described protocol.
- Validated the system's utility for proteins with predicted interactions.
Conclusions:
- The presented yeast-based system offers an efficient method for detecting protein-protein interactions.
- This protocol can be applied to study protein complex formation in various biological contexts.
Related Concept Videos
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
02:04Protein-protein Interfaces
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...