Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Trapping fish genes with transposons.

Darius Balciunas1, Stephen C Ekker

  • 1Arnold and Mabel Beckman Center for Transposon Research, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Zebrafish
|February 6, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recombinant ubbR promoter enables highly efficient tamoxifen-inducible Cre recombination in embryonic and adult zebrafish.

Genetics·2026
Same author

Validation and analysis of 12,000 AI-driven CAR-T designs in the <i>Bits to Binders</i> competitions.

bioRxiv : the preprint server for biology·2026
Same author

EpicTope: predicting and validating non-disruptive epitope tagging sites.

Development (Cambridge, England)·2026
Same author

A new role for PHYHD1 and related dioxygenases: demethylation of 2'-O-methylated nucleosides.

Nucleic acids research·2025
Same author

Epicardial Tcf21 facilitates cardiomyocyte dedifferentiation and heart regeneration in zebrafish.

Developmental biology·2025
Same author

Epigenome-wide association study of nuclear DNA methylation in relation to mitochondrial heteroplasmy.

Nature communications·2025
Same journal

Essential Oil of <i>Varronia curassavica</i> (Cordiaceae) as an Anesthetic and Sedative for Zebrafish.

Zebrafish·2026
Same journal

Dynamic Population Breeding: A Structured Colony Management Strategy to Improve Reproductive Performance and Early Survival in <i>Nothobranchius furzeri</i>.

Zebrafish·2026
Same journal

Auxin Inducible Protein Degradation in Zebrafish.

Zebrafish·2026
Same journal

<i>Corrigendum to:</i> A Zebrafish Heart Failure Model for Assessing Therapeutic Agents.

Zebrafish·2026
Same journal

Global Landscape of Zebrafish Research (2000-2025): A Bibliometric Analysis of Publication Trends, Collaboration, and Thematic Evolution.

Zebrafish·2026
Same journal

Lactic-Acid-Induced Zebrafish Skin Apoptosis as a Model for Evaluating Skin Barrier Function.

Zebrafish·2026
See all related articles

New fish models with tissue-specific GFP expression patterns are being developed using enhancer and gene trap methodologies. These tools enhance the value of fish for exploring novel biological phenomena in developmental genetics.

Area of Science:

  • Developmental genetics
  • Molecular biology
  • Transposon systems

Background:

  • Pilot screens have established enhancer and gene trap methodologies for use in fish.
  • These approaches have been validated by characterizing genes with novel expression patterns.

Purpose of the Study:

  • To summarize recent advancements in enhancer and gene trap methodologies in fish.
  • To compare the first generation of trap vectors.
  • To discuss future perspectives for these techniques.

Main Methods:

  • Utilizing Tol2 and Sleeping Beauty transposon systems for vector construction.
  • Developing tissue-specific Green Fluorescent Protein (GFP) expression patterns.
  • Characterizing gene expression patterns in established fish lines.

Related Experiment Videos

Main Results:

  • Successful adaptation of Tol2 and Sleeping Beauty transposon systems for trap vector construction.
  • Generation of fish lines with tissue-specific GFP expression.
  • Identification of genes with novel and unexpected expression patterns.

Conclusions:

  • Enhancer and gene trap methodologies are valuable tools for developmental genetics in fish.
  • The developed fish models enhance the exploration of novel biological phenomena.
  • Future work should address challenges for wider adoption of these methodologies.