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Published on: October 9, 2013
A Method for Measuring Intraocular Pressure in Wild-Type Zebrafish Using Rebound Tonometry: Characterization, Sex
Hsin-Lin Cheng1, Pei-Hsuan Chou1, Yung-Chang Yen2,3
1Department of BioMedical Sciences, Chung Shan Medical University, Taichung, Taiwan.
Zebrafish
|August 11, 2026
Summary
This study demonstrates that a handheld rebound tonometer reliably measures intraocular pressure (IOP) in zebrafish over 18 months. Zebrafish provide a consistent and reproducible vertebrate model for IOP research.
Area of Science:
- Ophthalmology
- Comparative Medicine
- Animal Models
Background:
- Intraocular pressure (IOP) monitoring is crucial for diagnosing and managing ocular diseases.
- A reliable vertebrate model for IOP characterization is needed for research.
Purpose of the Study:
- To investigate the use of a handheld rebound tonometer for stable IOP measurement in zebrafish.
- To characterize baseline IOP values in a zebrafish model over an extended period.
Main Methods:
- Wild-type zebrafish IOP was measured using the iCare PRO rebound tonometer over 18 months.
- Measurements were taken consistently at the same time points for both eyes (OD and OS).
Main Results:
- Long-term IOP values in male zebrafish (3-21 months) ranged from 21.96 to 23.39 mm Hg.
- Female zebrafish exhibited similar IOP ranges (22.10 to 23.19 mm Hg).
- No significant differences in IOP were found based on gender, time point, or between eyes (p > 0.05).
Conclusions:
- The iCare tonometer provides consistent and reproducible IOP measurements in zebrafish.
- Zebrafish are a reliable vertebrate model for long-term IOP studies.
- The established methodology ensures measurement reliability and consistency.
