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RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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Finding the shortest path in the shortest time using PCNN's.

H J Caulfield1, J M Kinser

  • 1Fisk University, Nashville, TN 37208-3051, USA.

IEEE Transactions on Neural Networks
|February 7, 2008
PubMed
Summary

Pulse coupled neural networks (PCNNs) explore all maze paths simultaneously. This allows them to efficiently find the shortest route in the minimum possible time.

Area of Science:

  • Computational neuroscience
  • Artificial intelligence
  • Network dynamics

Background:

  • Pulse coupled neural networks (PCNNs) are biologically inspired computational models.
  • Traditional algorithms for maze solving can be time-consuming and computationally intensive.

Purpose of the Study:

  • To investigate the capability of PCNNs for solving mazes.
  • To demonstrate the efficiency of PCNNs in finding the shortest path.

Main Methods:

  • Implementing a PCNN model capable of exploring multiple paths concurrently.
  • Simulating the network's response to maze traversal tasks.

Main Results:

  • PCNNs exhibit non-deterministic maze running, exploring all possible paths.

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  • The network identifies the shortest path in the shortest time due to its parallel processing nature.
  • Conclusions:

    • PCNNs offer a novel and efficient approach to maze-solving problems.
    • The parallel processing inherent in PCNNs is advantageous for optimization tasks.