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Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping
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Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping

Published on: September 4, 2009

Cultures of hESM human embryonic stem cells: chromosomal aberrations and karyotype stability

M A Prokhorovich1, M A Lagar'kova, A G Shilov

  • 1Novosibirsk State University, Novosibirsk.

Bulletin of Experimental Biology and Medicine
|February 8, 2008
PubMed

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Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...