[Separation of paroxetine and its intermediate enantiomers by high performance liquid chromatography using
1Sichuan Industrial Institute of Antibiotic, Chengdu 610051, China. lu_tg@126.com
Abstract:
A high performance liquid chromatographic method to separate the respective enantiomers of paroxetine and its intermediate was developed using chiral mobile phase additive. Separation was performed on a Diamond C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase was 0.1% phosphate acid-methanol (65 : 35, v/v) containing 0.38 g/L carboxymethyl-beta-cyclodextrin and the pH was adjusted to 7.2 by triethylamine. The detection wavelength was set at 210 nm and the temperature was 25 degrees C. With this method, paroxetine's trans/cis isomers and their enantiomers as well as intermediate HFP's trans/cis isomers and their enantiomers were separated simultaneously. The method is simple, rapid with high resolutions.
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