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Updated: Jun 24, 2026

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes
Published on: May 19, 2019
Mammalian Respiratory Chain Complex Assemblies and Their Links to Mitochondria Stress-Induced Human Diseases
Runyu Guo1,2, Maojun Yang3
1Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.
Abstract:
Mitochondria are considered the central organelle in cellular energy metabolism and an integral platform for signal transduction. Respiratory chain complexes are the most abundant and critical protein machines in mitochondria. Thanks to advancing technologies such as cryo-EM, molecular dynamics simulation, and FRET-based live imaging, though still under hot debate, we have now gained a much deeper insight into the organization, regulation, and functional mechanism of the respiratory chain. Accordingly, developing novel compounds targeting mitochondria is particularly appealing, for mitochondria dysfunction might be the underlying cause of many annoying human diseases, including metabolic syndromes, neurodegenerative diseases, cardiovascular diseases, and tumors.
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