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Abnormal Proliferation

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In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
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In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

Foxp3+CD4+ T cell-mediated immunosuppression involves extracellular nucleotide catabolism.

Margaret S Bynoe1, Christophe Viret

  • 1College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA. msb76@cornell.edu

Trends in Immunology
|February 9, 2008
PubMed
Summary

Naturally arising suppressor T cells, Foxp3(+)CD4(+) T cells, control autoimmune responses. Novel research shows ectoenzymes CD39 and CD73 generate adenosine, contributing to their regulatory activity.

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Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
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Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

Related Experiment Videos

Last Updated: Jul 7, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
08:20

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
15:33

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

Area of Science:

  • Immunology
  • T cell biology
  • Autoimmunity

Background:

  • Foxp3(+)CD4(+) T cells are critical for suppressing autoimmune reactions.
  • Their suppressive functions involve anti-inflammatory factors like TGF-beta and IL-10.

Purpose of the Study:

  • To investigate the role of ectoenzymes CD39 and CD73 in the regulatory activity of Foxp3(+)CD4(+) T cells.
  • To identify novel mechanisms of T cell-mediated immunosuppression.

Main Methods:

  • Analysis of ectoenzyme expression and function in Foxp3(+)CD4(+) T cells.
  • Assessment of adenosine generation and its impact on T cell suppressive activity.

Main Results:

  • Ectoenzymes CD39 and CD73 were identified as key players in Foxp3(+)CD4(+) T cell function.
  • These enzymes contribute to immunosuppression through the generation of adenosine.

Conclusions:

  • CD39 and CD73 are novel contributors to the regulatory capacity of Foxp3(+)CD4(+) T cells.
  • Adenosine production by these ectoenzymes represents an important immunosuppressive mechanism.