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Related Concept Videos

Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which provide...
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Amino Acid Catabolism

Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Related Experiment Video

Updated: Jul 7, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

Metabolic labeling with amino acids.

J S Bonifacino1

  • 1National Institute of Child Health and Human Development, Bethesda, Maryland, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed
Summary

Metabolic labeling with radioactive amino acids like [35S]-methionine is a key technique for tracking protein synthesis and fate. This guide details pulse-labeling and pulse-chase experiments, including safe handling of radioactive materials.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Metabolic labeling is crucial for understanding protein dynamics.
  • Tracking proteins requires specific labeling methodologies.

Purpose of the Study:

  • To provide an update on pulse-labeling and pulse-chase experiments.
  • To offer guidance on using radiolabeled amino acids for protein studies.
  • To ensure safe handling of 35S-labeled compounds.

Main Methods:

  • Focus on pulse-labeling and pulse-chase experiments using [35S]-methionine.
  • Provides directions for labeling with alternative radiolabeled amino acids.
  • Includes guidance for safe use and handling of 35S-labeled compounds.

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Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
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Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Published on: September 18, 2013

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
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Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Published on: February 20, 2013

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Last Updated: Jul 7, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
11:31

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Published on: September 18, 2013

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
09:43

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Published on: February 20, 2013

Main Results:

  • Demonstrates the utility of [35S]-methionine for studying protein biosynthesis, processing, transport, secretion, and degradation.
  • Offers practical instructions for researchers new to these techniques.
  • Emphasizes safety protocols for working with radioactive isotopes.

Conclusions:

  • Metabolic labeling, particularly with [35S]-methionine, remains a fundamental technique in protein research.
  • Proper methodology and safety precautions are essential for successful and safe experiments.
  • This update serves as a valuable resource for researchers utilizing these labeling techniques.