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Updated: Jul 7, 2026

Dissection of the Endolymphatic Sac from Mice
Published on: March 29, 2021
Expression of the Na+-K+-2Cl- cotransporter in the rat endolymphatic sac
Kosuke Akiyama1, Takenori Miyashita, Terushige Mori
1Department of Otolaryngology, Faculty of Medicine, Kagawa University, Kita-gun Miki-cho Ikenobe 1750-1 , Kagawa 761-0793, Japan.
Abstract:
The endolymphatic sac (ES) is a part of the membranous labyrinth that contains the cochlea, vestibular organs, an d semicircular canals, and is believed to absorb endolymphatic fluid. Na(+)-K(+)-2Cl(-) (NKCC) is a cotransporter that occurs as two isoforms (NKCC-1 and NKCC-2). Especially, NKCC-2 is suggested to participate in ES endolymph absorption. In the present study, the expression and cellular localization of NKCC-1 and NKCC-2 in the rat ES wer e examined by RT-PCR and in situ hybridization, respectively. The findings indicate that both NKCC-1 and NKCC-2 are expressed in the rat ES and suggest that NKCC is involved in ES homeostasis. NKCC-2 may be particularly involved in endolymph absorption. This is the first report confirming NKCC expression in the ES.
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