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Related Experiment Video

Updated: Jul 7, 2026

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
12:16

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Published on: March 13, 2013

MDR1 expression identifies human melanoma stem cells.

Gilmor I Keshet1, Itamar Goldstein, Orit Itzhaki

  • 1Cancer Research Center, Sheba Medical Center, Tel Hashomer and Sackler School of Medicine, Tel Aviv University Tel Aviv, 52621, Israel.

Biochemical and Biophysical Research Communications
|February 19, 2008
PubMed
Summary
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Multidrug-resistance gene product 1 (MDR1)-expressing melanoma cells exhibit stem cell properties, including enhanced self-renewal and sphere formation. These MDR1(+) cells represent a potential target for novel anti-melanoma therapies.

Area of Science:

  • Oncology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • ABC transporters confer toxin resistance in stem cells.
  • Multidrug resistance gene 1 (MDR1) expression in human melanoma cells was investigated.
  • Co-expression of ABCB5, ABCC2, nanog, and human telomerase reverse transcriptase (hTERT) was observed in MDR1(+) melanoma cells.

Purpose of the Study:

  • To investigate the stem cell characteristics of MDR1(+) melanoma cells.
  • To assess the clonogenicity and self-renewal capacity of MDR1(+) melanoma cells.
  • To identify potential cellular targets for anti-melanoma therapies.

Main Methods:

  • Fluorescence-activated cell sorting (FACS) was used to isolate MDR1(+) and MDR1(-) melanoma cell populations.
  • Limiting dilution assays were performed to evaluate clonogenicity and self-renewal capacity.

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Last Updated: Jul 7, 2026

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Published on: March 13, 2013

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells
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  • Sphere formation assays under low attachment conditions were conducted.

    • Main Results:

    • MDR1(+) melanoma cells exhibited significantly higher self-renewal capacity compared to MDR1(-) cells.
    • MDR1(+) cells demonstrated an increased ability to form spheres, a characteristic of cancer stem cells.
    • The isolated MDR1(+) subpopulation displayed key stem cell features.

    Conclusions:

    • The MDR1(+) subpopulation of human melanoma cells represents melanoma stem cells.
    • These findings suggest MDR1(+) cells are a promising cellular target for future anti-melanoma treatment strategies.
    • Targeting MDR1(+) melanoma stem cells could lead to more effective therapies.