Label-free detection with micro optical fluidic systems (MOFS): a review

Area of Science:

• Biophysics
• Biomedical Engineering
• Microfluidics
• Optics

Background:

• Micro optical fluidic systems (MOFS), also known as optofluidics, merge optics and fluidics within a microsystem.
• These systems are crucial for novel functionalities and in-depth analysis in the biophysical domain.

Purpose of the Study:

• To review the state-of-the-art in micro optical fluidic systems (MOFS).
• To discuss the implementation of MOFS in biomedical engineering and their applications in biophysical studies.
• To highlight various optical detection techniques integrated into MOFS.

Main Methods:

• Review of existing literature on MOFS and optofluidics.
• Discussion of optical detection techniques including evanescent wave, surface plasmon resonance, and surface-enhanced Raman scattering.
• Exploration of MOFS applications in cell biophysics, such as cell mass and Young's modulus measurement.

Main Results:

• MOFS integrate diverse optical detection methods like evanescent wave, SPR, and SERS.
• These systems enable advanced biophysical studies, including cell mass and mechanical property measurements.
• Near-field optics implementation is a key aspect discussed.

Conclusions:

• MOFS represent a powerful platform for biophysical research and biomedical applications.
• The integration of advanced optical techniques enhances analytical capabilities in microfluidic devices.
• Optofluidics provides novel avenues for understanding cellular biophysics at the microscale.