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[Effect of luteolin on COX-2 and mPGES-1 expression in LPS-induced RAW264.7 cells]
Xu-guang Wang1, Gen-yin Chen, Miao-ping Chen
1First Affiliated Hospital of Guangdong Medical College, Zhanjiang 524100, China. marvin009@126.com
Objective:
To study the effect of luteolin on COX-2 and mPGES-1 expression in LPS-induced RAW264.7 cells.
Methods:
After being pretreated with different concentrations of luteolin for 30 min, and then incubated with 1 microg/ml LPS for 12h, the effect of luteolin on the product of PGE2 in RAW264.7 cells was measured by enzyme immunoassay (EIA). The mRNA expression of COX-2 and mPGES-1 in RAW264.7 cells were analysed by RT-PCR. The COX-2 and mPGES-1 protein expression in RAW264. 7 cells were analysed by western blotting.
Results:
Luteolin inhibited the LPS-induced PGE, synthesis in RAW264.7 cells. The mRNA and protein expression of COX-2 and mPGES-1 in RAW264.7 cells were also decreased by luteolin.
Conclusion:
Luteolin can inhibit significantly the expression of COX-2 and mPGES-1 in PGE2 synthetic pathway.
