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Updated: Jul 7, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
Published on: November 1, 2024
Sources of sensitivity losses in ultrafast 2D NMR
Patrick Giraudeau1, Serge Akoka
1Université de Nantes, Nantes Atlantique Universités, CNRS, Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes, UMR 6006, Faculté des Sciences, B.P. 92208, Nantes Cedex 03, France. patrick.giraudeau@univ-nantes.fr
Abstract:
Recent ultrafast techniques make it possible to obtain nD NMR spectra in a single scan. However, an important sensitivity decrease is observed when the excitation duration is increased, which is necessary to improve resolution. A detailed, theoretical and experimental study of sensitivity losses in ultrafast experiments is carried out on the example of J-resolved spectroscopy. The importance of molecular diffusion effects during both encoding and acquisition phases is shown by numerical simulations and experimental results. Other possible sources of signal-to-noise decrease are also considered, such as transverse relaxation, homonuclear J-couplings or chemical shift effects.
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