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[Molecular-biological identification of pathogens which caused an outbreak of viral encephalitis in Jinan area]

Yan-zhen Wan1, Ying-ying Yue, Peng Li

  • 1Department of Microbiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China.

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology
|March 7, 2008
PubMed
Abstract

Insights

Coxsackievirus B5 caused the 2003 viral encephalitis outbreak in Jinan. This study identified the virus strain, linking it to similar outbreaks in Zhejiang province.

Area of Science:

  • Virology
  • Molecular Biology
  • Epidemiology

Context:

  • An outbreak of viral encephalitis occurred in Jinan in 2003.
  • Enterovirus infections can cause severe neurological disease.

Purpose:

  • To determine the causative agent of the 2003 viral encephalitis outbreak in Jinan.
  • To identify the specific genotype of the identified virus.

Summary:

  • Five strains of Coxsackievirus B5 were isolated from clinical samples during the Jinan outbreak.
  • Genetic analysis confirmed the virus as Coxsackievirus B5, highly homologous to strains from Zhejiang province (2002-2004).
  • Techniques used included random PCR, RT-PCR, sequencing, BLAST analysis, and Clustal W alignment.

Impact:

  • Identifies Coxsackievirus B5 as a significant cause of the Jinan encephalitis outbreak.
  • Provides crucial etiological data for understanding enterovirus-related neurological diseases.
  • Highlights the potential for cross-regional viral transmission and outbreaks.

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