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[Study on HPLC fingerprint of processed Radix Angelicae Dahuricae]
Cui-ying Zhang1, Zhen-guo Li, Qing-xiao Wang
1Henan Provincial Institute for Food and Drug Control, Zhengzhou 450003, China.
Objective:
To establish HPLC fingerprint analysis for the quality control of processed Radix Angelicae Dahuricae.
Methods:
HPLC fingerprint analysis of processed Radix Angelicae Dahuricae was developed Agilent C18 column (250 mm x 4.6 mm, 5 microm) was used, with mixture of acetonitrile and 5% phosphorous acid mobile phase in a gradient mode. The flow rate was 1.0 mL/min. The wavelength of measurement was 254 nm. Fifteen batches of processed Radix Angelicae Dahuricae were determined.
Results:
The methodological evaluation showed that the method had a good repeatability.
Conclusion:
The method can be used to identify and evaluate the quality of Radix Angelicae Dahuricae conveniently.