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Updated: Jul 6, 2026
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
Comparison between E-test and CLSI broth microdilution method for antifungal susceptibility testing of Candida
Cristiane Yumi Koga-Ito1, Juliana Pereira Lyon, Maria Aparecida de Resende
1Departamento de Biociências e Diagnóstico Bucal, Universidade Estadual Paulista Júlio de Mesquita Filho, São José dos Campos, SP, Brazil.
Abstract:
Thirty Candida albicans isolated from oral candidosis patients and 30 C. albicans isolated from control individuals were studied. In vitro susceptibility tests were performed for amphotericin B, fluconazole, 5-flucytosine and itraconazole through the Clinical and Laboratorial Standards Institute (CLSI) reference method and E test system. The results obtained were analyzed and compared. MIC values were similar for the strains isolated from oral candidosis patients and control individuals. The agreement rate for the two methods was 66.67% for amphotericin B, 53.33% for fluconazole, 65% for flucytosine and 45% for itraconazole. According to our data, E test method could be an alternative to trial routine susceptibility testing due to its simplicity. However, it can not be considered a substitute for the CLSI reference method.
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