Related Experiment Video
Updated: Jul 6, 2026
09:05
In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
Published on: August 9, 2017
Common marmoset embryonic stem cell can differentiate into cardiomyocytes
Hao Chen1, Fumiyuki Hattori, Mitsushige Murata
1Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Biochemical and Biophysical Research Communications
|March 12, 2008
Summary
Common marmoset embryonic stem cell-derived cardiomyocytes exhibit functional characteristics and high proliferative potential. These cells show promise for advancing cardiovascular regenerative medicine research.
Area of Science:
- Stem Cell Biology
- Cardiovascular Research
- Primate Models
Background:
- Common marmoset embryonic stem cells (CMESC) are a valuable primate research model.
- Previous work established CMESC lines capable of differentiating into three germ layers.
Purpose of the Study:
- To investigate the differentiation potential of CMESC into cardiomyocytes.
- To characterize the functional properties and proliferative capacity of these derived cardiomyocytes.
Main Methods:
- Induction of differentiation from CMESC.
- Gene expression analysis (FOG-2, GATA4, Tbx20, Nkx2.5, Tbx5).
- Assessment of spontaneous beating, immunofluorescence, ultrastructural analysis, electrophysiology, and BrdU incorporation assays.
Main Results:
- CMESC successfully differentiated into cardiomyocytes, expressing key cardiac markers.
- Spontaneous beating observed from days 12-15, with functional cardiomyocyte characteristics confirmed.
- Derived cardiomyocytes exhibited sinus node-like action potentials and retained high proliferative potential up to 24 weeks.
Conclusions:
- CMESC-derived cardiomyocytes possess functional and proliferative characteristics suitable for research.
- These cells represent a significant advancement for preclinical cardiovascular regenerative medicine studies.
- The common marmoset model offers unique advantages for cardiac research.
Related Concept Videos
00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
01:22iPS Cell Differentiation
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
01:27Source And Potency Of Stem Cells
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
01:19Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...