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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: Jul 6, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

A dual-tag microarray platform for high-performance nucleic acid and protein analyses.

Olle Ericsson¹, Jonas Jarvius, Edith Schallmeiner

¹Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala University, SE-75185 Uppsala, Sweden.

Nucleic Acids Research

|March 19, 2008

Summary

This summary is machine-generated.

This study introduces a high-performance tag microarray method for sensitive DNA detection. The new procedure enhances specificity and dynamic range, enabling analysis of minimal target molecules for gene and protein expression studies.

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Published on: July 21, 2014

Area of Science:

Molecular Biology
Biotechnology
Genomics

Background:

DNA microarrays are essential for monitoring molecular events.
Current microarray limitations include specificity and dynamic range issues, hindering detection of weakly expressed genes and proteins.

Purpose of the Study:

To develop a high-performance tag microarray procedure for enhanced sensitivity and specificity.
To extend the utility of DNA microarray approaches for analyzing low-abundance molecules and protein interactions.

Main Methods:

Developed a tag microarray procedure enabling probe-based analysis of as few as 100 target cDNA molecules.
Incorporated single-molecule analysis and real-time monitoring of rolling-circle amplification.
Utilized padlock and proximity probe ligation to convert mRNA and proteins into tag DNA sequences.

Main Results:

Achieved a linear dynamic range close to 10^5.
Significantly decreased the risk of cross-hybridization compared to existing methods.
Demonstrated proof of concept for measuring mRNA and proteins using the microarray technique.

Conclusions:

The high-performance tag microarray procedure offers enhanced sensitivity and specificity for molecular detection.
This method expands the capabilities of DNA microarrays for analyzing low-abundance targets and protein interactions.
The protocol facilitates single-molecule quantification and real-time monitoring, advancing molecular diagnostics and research.