Concentration changes to counteract the effects of bacteriological sampling on PLT yields

Area of Science:

• Transfusion Medicine
• Hematology

Background:

• Bacterial culturing of apheresis platelet (PLT) units was associated with increased low yield products (LYP) and decreased multiple PLT products.
• A retrospective analysis was conducted to evaluate the impact of sampling and subsequent collection modifications on PLT yield and adverse reactions.

Purpose of the Study:

• To determine the effects of sampling and modifications in apheresis collections on platelet yield and adverse reactions.
• To assess strategies for mitigating the negative impacts of bacterial culturing on PLT collection efficiency.

Main Methods:

• A retrospective analysis of four time periods: baseline, sampling implementation, concentration change (Conc. Delta), and concentration and yield target change (Target + Conc. Delta).
• Collections were performed using the Gambro Trima Accel, with adjustments to PLT concentration settings and yield targets.
• PLT concentration was reduced, and specific yield targets were modified (elimination of 3.2 x 10(11) and addition of 9.4 x 10(11)).

Main Results:

• The incidence of LYP significantly increased with sampling but declined significantly after concentration and target modifications.
• The average number of PLT products per donor per week was maintained or increased with the Target + Conc. Delta strategy.
• The incidence of triple PLT products significantly increased with the Target + Conc. Delta strategy compared to baseline and sampling groups.
• No significant differences in donor or recipient reaction incidence were observed across the groups.

Conclusions:

• Modifications in yield targets and PLT concentration effectively compensated for the increased incidence of low yield products associated with bacterial culturing.
• These changes optimized PLT collection efficiency without adversely affecting donor or recipient safety.