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Updated: Jul 6, 2026
10:05
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Multi-dimensional capillary electrophoresis and chromatography for proteomic analysis
1Department of Chemistry & Research Center for Proteome, Fudan University, Shanghai, China.
Methods in Molecular Biology (Clifton, N.J.)
|April 9, 2008
Summary
This chapter reviews comprehensive two-dimensional liquid chromatography-capillary electrophoresis systems. Applications include proteomic analysis and single-cell protein expression profiling using advanced hyphenated techniques.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Separation Science
Background:
- Comprehensive two-dimensional liquid chromatography-capillary electrophoresis (2D LC-CE) offers enhanced separation power.
- Various combinations of LC and CE modes, interfaces, and detection technologies are crucial for complex analyses.
Purpose of the Study:
- To summarize existing comprehensive 2D LC-CE systems.
- To highlight their applications in proteomics and single-cell analysis.
Main Methods:
- Review of diverse 2D LC-CE configurations.
- Demonstration of a reverse-phase LC-fast CE-MS system for proteomics.
- Presentation of a 2D CE system coupling capillary sieving electrophoresis with micellar electrokinetic chromatography for single-cell analysis.
Main Results:
- Successful application of 2D LC-CE-MS in proteomic analysis.
- Effective use of coupled CE techniques for single-cell protein expression profiling.
Conclusions:
- Comprehensive 2D LC-CE systems provide powerful analytical platforms.
- These systems are valuable for high-resolution separation and sensitive detection in complex biological samples.
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