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Flow methodology for methanol determination in biodiesel exploiting membrane-based extraction.

André R T S Araujo¹, M Lúcia M F S Saraiva, José L F C Lima

¹REQUIMTE, Departamento de Química-Física, Faculdade de Farmácia da Universidade do Porto, Rua Aníbal Cunha 164, 4099-030 Porto, Portugal.

Analytica Chimica Acta

|April 9, 2008

Summary

This study introduces a new flow analysis and membrane extraction method for quantifying methanol in biodiesel. The environmentally friendly technique offers accurate results comparable to gas chromatography.

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Area of Science:

Analytical Chemistry
Environmental Science
Biotechnology

Background:

Accurate determination of methanol in biodiesel is crucial for quality control and regulatory compliance.
Existing methods, such as gas chromatography, can be time-consuming and costly.
There is a need for more efficient, cost-effective, and environmentally friendly analytical techniques.

Purpose of the Study:

To develop and optimize a flow analysis and membrane-based extraction methodology for methanol determination in biodiesel.
To evaluate the performance of the developed method in terms of sensitivity, precision, and accuracy.
To compare the proposed method with the established gas chromatography reference method.

Main Methods:

Liquid-liquid extraction using a hydrophilic membrane with biodiesel as the donor phase and an aqueous buffer as the acceptor phase.
Quantification of methanol in the aqueous phase using immobilized alcohol oxidase (AOD), peroxidase, and ABTS.
Optimization of key parameters including membrane type, reactor design, and acceptor solution volume.

Main Results:

Two dynamic analytical ranges were achieved (up to 0.015% and 0.200% m/m methanol) by adjusting the acceptor solution volume.
Low detection limits (0.0002% and 0.007% m/m) and high precision (R.S.D. <5.0%) were demonstrated.
The method showed good agreement with gas chromatography for real biodiesel samples, requiring no pre-treatment.

Conclusions:

The developed flow analysis and membrane extraction method provides a reliable and sensitive approach for methanol quantification in biodiesel.
The methodology is more environmental friendly and cost-effective compared to the gas chromatography reference method.
This technique offers a promising alternative for routine analysis of methanol in biodiesel.