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CD3 expression, modulation and signalling in T-cell subpopulations from MRL/Mp-lpr/lpr mice
J L Davignon1, L W Arnold, P L Cohen
1Department of Medicine, University of North Carolina, Chapel Hill 27599.
Journal of Autoimmunity
|December 1, 1991
Summary
Abnormal T cells in lpr mice show altered CD3 expression and impaired calcium signaling. These findings highlight unique T-cell abnormalities in lpr mice, impacting immune responses.
Area of Science:
- Immunology
- Cell Biology
Background:
- MRL/Mp-lpr2lpr mice exhibit an expanded and abnormal T-cell population.
- Previous studies indicated aberrant T-cell receptor expression and modulation in CD4- CD8- (DN) T cells.
Purpose of the Study:
- To further investigate T-cell abnormalities in lpr mice.
- To examine CD3 expression and modulation in T-cell subpopulations.
- To measure calcium (Ca++) mobilization in DN lpr T cells.
Main Methods:
- Analysis of CD3 expression and modulation in CD4hi, CD8hi, CD4lo, and CD8lo T-cell subsets from +/+ and lpr mice.
- Measurement of mitogen-induced Ca++ mobilization in DN lpr T cells using concanavalin A and anti-CD3 antibody.
Main Results:
- CD3 expression and modulation in CD4hi and CD8hi lpr T cells were similar to +/+ T cells.
- Identified CD4lo and CD8lo lpr T-cell subsets with intermediate CD3 expression and modulation.
- Observed impaired Ca++ mobilization in DN lpr T cells in response to stimuli.
Conclusions:
- CD4lo/CD8lo T-cell subsets may represent a transitional stage between single positive (SP) and DN populations.
- Rapid CD3 modulation in DN lpr T cells may be a cause, not a result, of low CD3 expression.
- These findings further characterize the distinct T-cell abnormalities present in lpr mice, impacting immune function.