Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Method for macromolecular colocalization using atomic recombination in dynamic SIMS.

G Legent1, A Delaune, V Norris

  • 1Laboratoire Assemblages moléculaires: modélisation, et imagerie SIMS, Faculté des Sciences de l'Université de Rouen, 76821 Mont Saint Aignan Cedex, France.

The Journal of Physical Chemistry. B
|April 11, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three-dimensional markerless pose estimation for anatomical landmarks of the shoulder and upper limb.

Journal of electromyography and kinesiology : official journal of the International Society of Electrophysiological Kinesiology·2025
Same author

A Closed Circulation Langendorff Heart Perfusion Method for Cardiac Drug Screening.

Physiological research·2025
Same author

Early Dynamics of Portal Pressure Gradient After TIPS Insertion Predict Mortality.

Alimentary pharmacology & therapeutics·2025
Same author

[Cataract surgery training in France: Analysis of the results of the European Board of Ophthalmology survey in the French cohort].

Journal francais d'ophtalmologie·2024
Same author

Training in cataract surgery in Spain: analysis of the results of a survey of the European Board of Ophthalmology in a Spanish cohort.

Archivos de la Sociedad Espanola de Oftalmologia·2024
Same author

Pregnancy outcomes in women with imported malaria in mainland France: A retrospective study from 2004 to 2014.

Travel medicine and infectious disease·2024
Same journal

From Cation Solvation to Anion Coordination: Lewis-Acidic Boranes Enable Halide Salt Electrolytes.

The journal of physical chemistry. B·2026
Same journal

In Vitro-Prepared A30P Alpha-Synuclein Fibrils Adopt the Conserved and Disease-Relevant Greek Key Fold.

The journal of physical chemistry. B·2026
Same journal

Metastructure Analysis of Self-Assembled Nanocubes with Different Equatorial Methyl Groups Based on Molecular Dynamics Simulations.

The journal of physical chemistry. B·2026
Same journal

A Cocoordinated <sup>1</sup>H Internal Reference Quantifies Proton-Exchange Bias in Coordinated-Water Diffusion.

The journal of physical chemistry. B·2026
Same journal

Unveiling Electrolyte-Dependent Coordination Site Dynamics for Redox Mediator Design in Lithium-O<sub>2</sub> Batteries: Exchange vs Rearrangement.

The journal of physical chemistry. B·2026
Same journal

The Role of Functional Groups in Substituted Benzoic Acids Used as Dopants in Liquid Crystal Mixtures on the Nematic-Isotropic Transitions.

The journal of physical chemistry. B·2026
See all related articles

A new technique using dynamic secondary ion mass spectrometry (D-SIMS) allows researchers to colocalize macromolecules within 2 nanometers. This method exploits the formation of recombinant CN secondary ions for precise biological imaging.

Area of Science:

  • Biophysics
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Localizing biological components requires advanced imaging techniques.
  • Dynamic Secondary Ion Mass Spectrometry (D-SIMS) offers high resolution and sensitivity for molecular localization.

Purpose of the Study:

  • To develop a novel colocalization technique using D-SIMS.
  • To assess the feasibility of using recombinant CN secondary ion formation for nanoscale molecular proximity analysis.

Main Methods:

  • Utilized the Cameca NanoSIMS 50 for D-SIMS analysis.
  • Employed isotopically labeled glycine crystals and protein mixtures.
  • Developed a convolution model for quantitative interpretation of D-SIMS data.

Main Results:

Related Experiment Videos

  • Demonstrated that recombinant (13)C(15)N ion formation indicates macromolecular distance and shape.
  • Established that macromolecules within 2 nm can be colocalized.
  • Validated the new D-SIMS-based colocalization technique.

Conclusions:

  • The novel D-SIMS technique enables precise colocalization of macromolecules at the nanoscale.
  • This method holds significant potential for advancing biological imaging and molecular interaction studies.