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Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
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Transgenic Organisms

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Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...

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Related Experiment Video

Updated: Jul 5, 2026

Electroporation of Plasmid DNA into Mouse Skeletal Muscle
06:20

Electroporation of Plasmid DNA into Mouse Skeletal Muscle

Published on: April 6, 2022

Gene delivery to muscle.

Matthew L Springer1, Thomas A Rando, Helen M Blau

  • 1Stanford University School of Medicine, Stanford, California, USA.

Current Protocols in Human Genetics
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

Gene delivery to skeletal muscle via myoblast implantation, DNA injection, or viral transduction offers therapeutic potential for neuromuscular disorders. This unit details methods for mouse myoblast transduction, DNA/myoblast injection, and muscle analysis.

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Last Updated: Jul 5, 2026

Electroporation of Plasmid DNA into Mouse Skeletal Muscle
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Published on: March 27, 2013

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Gene delivery to skeletal muscle is crucial for treating neuromuscular and systemic disorders.
  • Transgenic and knockout mouse models are vital for studying these conditions.
  • Current methods require efficient and reliable gene transfer techniques.

Purpose of the Study:

  • To describe established protocols for gene delivery into mouse skeletal muscle.
  • To provide detailed methods for myoblast isolation, retroviral transduction, and injection.
  • To outline histological analysis techniques for evaluating gene transfer efficacy.

Main Methods:

  • Isolation and retroviral transduction of mouse myoblasts.
  • Intramuscular injection of myoblasts and plasmid DNA.
  • Histological examination of recipient muscle tissue.
  • Plasmid DNA injection with or without electro-transfer.

Main Results:

  • Successful isolation and transduction of mouse myoblasts.
  • Demonstrated efficacy of myoblast and DNA injection into mouse muscle.
  • Established histological methods for assessing gene delivery outcomes.
  • Validated plasmid DNA delivery with and without electric charge.

Conclusions:

  • Myoblast implantation, DNA injection, and viral transduction are effective gene delivery strategies for skeletal muscle.
  • The described methods provide a comprehensive toolkit for genetic manipulation of mouse muscle.
  • These techniques are applicable to the study and potential treatment of neuromuscular diseases using mouse models.