Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential display of mRNA by PCR.

Current protocols in human genetics·2008
Same author

FLRT3 is expressed in sensory neurons after peripheral nerve injury and regulates neurite outgrowth.

Molecular and cellular neurosciences·2004
Same author

Strategies for microarray analysis of limiting amounts of RNA.

Briefings in functional genomics & proteomics·2004
Same author

An analysis of the gene expression program of mammalian neural progenitor cells.

Proceedings of the National Academy of Sciences of the United States of America·2004
Same author

Vertebrate neural cell-fate determination: lessons from the retina.

Nature reviews. Neuroscience·2001
Same author

Netrins and netrin receptors.

Cellular and molecular life sciences : CMLS·2001

Related Experiment Video

Updated: Jul 5, 2026

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
07:06

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

Differential display of mRNA by PCR.

F J Livesey1, C P Hunt

  • 1Trinity College, Dublin, Ireland.

Current Protocols in Neuroscience
|April 23, 2008
PubMed
Summary

This study details a modified mRNA differential display technique using polymerase chain reaction (PCR) to find genes with varying expression levels between cells. It covers gene identification, reamplification, cloning, and sample preparation for accurate gene expression analysis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Gene expression analysis is crucial for understanding cellular function and disease.
  • Traditional methods for identifying differentially expressed genes can be complex and time-consuming.

Purpose of the Study:

  • To present a modified single base-anchored mRNA differential display technique.
  • To provide a detailed protocol for identifying and isolating differentially expressed genes.

Main Methods:

  • Utilizes polymerase chain reaction (PCR) for mRNA differential display.
  • Includes steps for removing genomic DNA contamination from RNA samples.
  • Describes reverse transcription to create complementary DNA (cDNA) for PCR.

Main Results:

More Related Videos

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
07:26

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Published on: July 29, 2019

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

Related Experiment Videos

Last Updated: Jul 5, 2026

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
07:06

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
07:26

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Published on: July 29, 2019

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

  • The protocol facilitates the identification of candidate differentially expressed genes.
  • Details are provided for the reamplification and cloning of these candidate genes.
  • The method allows for the detection of gene expression differences between various cell or tissue types.

Conclusions:

  • The described single base-anchored differential display PCR is an effective method for discovering novel genes with differential expression.
  • This technique aids in the molecular characterization of cells and tissues by highlighting unique gene expression profiles.