Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reducing gene expression in the brain via antisense methods.

A M Ouagazzal1, J M Tepper, I Creese

  • 1United Medical and Dental Schools, Guy's Hospital, London, UK.

Current Protocols in Neuroscience
|April 23, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cholinergic interneuron control of GABAergic circuits targeting spiny projection neurons is disrupted in parkinsonian models.

bioRxiv : the preprint server for biology·2026
Same author

A history of chronic morphine exposure during adolescence increases despair-like behaviour and strain-dependently promotes sociability in abstinent adult mice.

Behavioural brain research·2013
Same author

Modulation of alpha-1 adrenergic receptors in rat brain following chronic reserpine.

Neurochemistry international·2010
Same author

Regulation of putative muscarinic cholinergic receptor subtypes in rat brain.

Neurochemistry international·2010
Same author

Basal ganglia macrocircuits.

Progress in brain research·2007
Same author

Pallidal control of substantia nigra dopaminergic neuron firing pattern and its relation to extracellular neostriatal dopamine levels.

Neuroscience·2004
Same journal

Simultaneous Ca<sup>2+</sup> Imaging and Optogenetic Stimulation of Cortical Astrocytes in Adult Murine Brain Slices.

Current protocols in neuroscience·2020
Same journal

Automated Two-Chamber Operon ID/ED Task for Mice.

Current protocols in neuroscience·2020
Same journal

A Guide to Fluorescence Lifetime Microscopy and Förster's Resonance Energy Transfer in Neuroscience.

Current protocols in neuroscience·2020
Same journal

Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research.

Current protocols in neuroscience·2020
Same journal

Recombinant Antibodies in Basic Neuroscience Research.

Current protocols in neuroscience·2020
Same journal

Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model.

Current protocols in neuroscience·2020
See all related articles

Antisense oligodeoxynucleotides can reduce brain protein expression. Direct delivery into the cerebrospinal fluid (CSF) or brain parenchyma overcomes poor central nervous system (CNS) penetration for targeted protein inhibition.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pharmacology

Background:

  • Antisense oligodeoxynucleotides (ASOs) are tools for reducing target protein expression.
  • ASOs function by inhibiting protein synthesis via mRNA hybridization.
  • Systemic administration of ASOs results in poor penetration into the central nervous system (CNS).

Purpose of the Study:

  • To present protocols for delivering antisense oligodeoxynucleotides into the brain.
  • To detail methods for reducing target protein expression in the CNS using ASOs.

Main Methods:

  • Continuous delivery of ASOs into the cerebrospinal fluid (CSF).
  • Continuous infusion of ASOs directly into brain parenchyma, exemplified by the substantia nigra.

Main Results:

Related Experiment Videos

  • Established protocols for effective ASO delivery to the CNS.
  • Demonstrated methods for targeted protein reduction in specific brain regions.

Conclusions:

  • Direct CNS delivery of ASOs is a viable strategy for modulating protein expression.
  • These protocols enable targeted therapeutic interventions within the brain.