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Updated: Jul 5, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
Published on: August 20, 2014
Use of fluorescence spectroscopy to elucidate RNA folding pathways
Philip C Bevilacqua1, Douglas H Turner
1The Pennsylvania State University, University Park, Pennsylvania, USA.
Abstract:
This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast kinetic processes with real-time data acquisition. The unit discusses the advantages and disadvantages of various pendant probes and nucleotide analogs, the analytical methods that can be used, instrument setup, control experiments, and a variety of kinetic experiments that can be performed, such as determination of rate constants.
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