Solid-phase synthesis of 2'-deoxy-2'-fluoro- beta-D-oligoarabinonucleotides (2'F-ANA) and their phosphorothioate
Ekaterina Viazovkina1, Maria M Mangos, Mohamed I Elzagheid
1McGill University, Montreal, Canada.
Current Protocols in Nucleic Acid Chemistry
|April 23, 2008
Abstract:
This unit describes the chemical synthesis of 2'-deoxy-2'-fluoro-b-D-oligoarabinonucleotides (2'F-ANA), both with phosphodiester and phosphorothioate linkages. The protocols described herein include araF phosphoramidite preparation, assembly on DNA synthesizers, and final deprotection and purification of oligonucleotides.
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