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Measuring protein interactions by optical biosensors
P Schuck1, L F Boyd, P S Andersen
1National Institutes of Health, Bethesda, Maryland, USA.
Abstract:
This unit gives an introduction to the basic techniques of optical biosensing for measuring equilibrium and kinetics of reversible protein interactions. Emphasis is given to the description of simple and robust approaches that will provide reliable results. How to avoid the most commonly encountered problems and artifacts is also discussed.
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