Related Experiment Video
Updated: Jul 5, 2026
07:27
Murine Mesenteric Lymphadenectomy for Selective Disruption of Lymphatic Communication with Region-Specific Gut
Published on: December 30, 2025
Removal of lymphoid organs.
1Food and Drug Administration, Bethesda, Maryland, USA.
Current Protocols in Immunology
|April 25, 2008
Summary
This study details survival surgery techniques for mouse thymus and spleen removal. These methods allow animals to recover for further research, unlike traditional tissue harvesting.
Area of Science:
- Immunology
- Surgical Techniques
- Animal Research Models
Background:
- Standard experimental procedures often involve harvesting lymphoid tissues from euthanized animals.
- Certain research questions necessitate the use of live animals post-lymphoid tissue removal for longitudinal studies.
Purpose of the Study:
- To describe established survival surgical procedures for the removal of the thymus and spleen in mice.
- To provide a methodology for researchers requiring intact, recovering animal models after specific lymphoid organ extirpation.
Main Methods:
- Detailed description of aseptic survival surgery for adult mouse spleen removal.
- Methodology for adult and neonatal mouse thymus removal under anesthesia.
- Post-operative care and animal recovery protocols.
Main Results:
- Successful surgical techniques for the extirpation of the spleen and thymus in mice are presented.
- The described methods facilitate animal survival and recovery for subsequent experimental phases.
Conclusions:
- Survival surgery for thymus and spleen removal in mice is a viable technique.
- These procedures support advanced research designs requiring post-operative animal viability for immunological or other investigations.
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