Related Experiment Video
Updated: Jul 5, 2026
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
Published on: January 13, 2016
Transfection of lymphoid cells
1University of California Los Angeles, Los Angeles, California, USA.
Current Protocols in Immunology
|April 25, 2008
Summary
Researchers can transfer immunoglobulin heavy (H) and light (L) chains into myeloma cells using various transfection methods. Protoplast fusion, electroporation, and liposome-mediated transfection are discussed for stable cell line development.
Area of Science:
- Molecular Biology
- Cell Biology
- Immunology
Background:
- Stable myeloma cell lines are crucial for immunoglobulin research.
- Efficient methods for transferring immunoglobulin genes are needed.
Purpose of the Study:
- To describe methods for simultaneous transfer of immunoglobulin heavy (H) and light (L) chains into myeloma cells.
- To compare different techniques for achieving stable transfection.
Main Methods:
- Protoplast fusion for lymphoid cell transfection.
- Electroporation for lymphoid cell transfection.
- Liposome-mediated transfection for lymphoid cell transfection.
Main Results:
- Successful simultaneous transfer of single or both immunoglobulin chains (H and L) is achievable.
- Protoplast fusion is suitable for specific lymphoid cells but can be cumbersome.
- Electroporation and liposome-mediated transfection offer alternatives.
Conclusions:
- Multiple methods exist for stable immunoglobulin gene transfer into myeloma cells.
- Electroporation and liposome-mediated transfection are generally less cumbersome than protoplast fusion.
- These transfection techniques can be adapted for other eukaryotic cell lines.
