Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

cDNA amplification using one-sided (anchored) PCR.

R L Dorit¹, O Ohara

¹Yale University, New Haven, Connecticut, USA.

Current Protocols in Immunology

|April 25, 2008

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Glial reactivity and cognitive decline follow chronic heterochromatin loss in neurons.

Nature communications·2025

Same author

Analysis of Mutations in the IL2RG Gene in 2 Asian Infants With X-linked Severe Combined Immunodeficiency.

Journal of investigational allergology & clinical immunology·2015

Same author

Successful treatment of systemic cytomegalovirus infection in severe combined immunodeficiency using allogeneic bone marrow transplantation followed by adoptive immunotherapy.

Journal of investigational allergology & clinical immunology·2014

Same author

Chronic granulomatous disease: two decades of experience from a tertiary care centre in North West India.

Journal of clinical immunology·2013

Same author

Novel mutation of IL2RG gene in a Korean boy with X-linked severe combined immunodeficiency.

Journal of investigational allergology & clinical immunology·2013

Same author

Severe primary antibody deficiency due to a novel mutation of mu heavy chain.

Journal of investigational allergology & clinical immunology·2012

Same journal

A Comprehensive Experimental Guide to Studying Cross-Presentation in Dendritic Cells In Vitro.

Current protocols in immunology·2020

Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020

Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020

Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020

Same journal

Monoclonal Antibodies to Human Cell Surface Antigens.

Current protocols in immunology·2020

Same journal

Protocols for Experimental Sjögren's Syndrome.

Current protocols in immunology·2020

See all related articles

Anchored PCR amplifies full-length mRNA using limited sequence data. This technique employs oligo(dT) primers for targeted amplification, enabling direct sequencing or cloning of mRNA molecules.

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Amplifying full-length mRNA is crucial for gene expression studies.
Limited sequence information often hinders traditional mRNA amplification methods.

Purpose of the Study:

To present anchored PCR, a modified PCR technique for full-length mRNA amplification.
To enable amplification using minimal known mRNA sequence data.

Main Methods:

Anchored PCR utilizes oligo(dT) primers for amplification.
Primers bind to either the poly(A) tail or an enzymatically added homopolymer tail.
Two rounds of PCR amplification are performed.

Main Results:

Achieved amplification of full-length mRNA from limited sequence information.

Related Experiment Videos

Generated a single, amplicon product suitable for downstream applications.

Conclusions:

Anchored PCR is an effective method for full-length mRNA amplification.
The technique facilitates direct sequencing or cloning of amplified mRNA.