Related Experiment Video
Updated: Jul 5, 2026
09:12
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
Published on: October 17, 2018
Characterization of human monocytes/macrophages
Mary C Riedy1, Carleton C Stewart1
1Roswell Park Cancer Institute, Buffalo, New York.
Current Protocols in Immunology
|April 25, 2008
Summary
Monoclonal antibodies (MAbs) identify cell surface markers on human monocytes and macrophages. Flow cytometry protocols are detailed for immunophenotypic characterization, aiding in understanding cell development and activation status.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Human monocytes and macrophages express diverse cell-surface determinants.
- Monoclonal antibodies (MAbs) are crucial tools for identifying these determinants.
- Understanding monocyte/macrophage phenotypes provides insights into their function.
Purpose of the Study:
- To describe flow cytometry methods for analyzing monocyte/macrophage cell-surface markers.
- To detail protocols for using directly conjugated and unconjugated MAbs.
- To facilitate immunophenotypic characterization using three-color analysis.
Main Methods:
- Flow cytometry protocols for measuring membrane determinant expression.
- Application of directly conjugated and unconjugated MAbs.
- Three-color flow cytometry analysis using combinations of MAbs.
Main Results:
- Established protocols for flow cytometry analysis of monocytes/macrophages.
- Demonstrated utility of MAbs for phenotypic profiling.
- Provided guidance on MAb combinations for detailed immunophenotyping.
Conclusions:
- Flow cytometry with MAbs is effective for characterizing monocyte/macrophage phenotypes.
- Three-color analysis enhances immunophenotypic resolution.
- This methodology aids in studying monocyte/macrophage development and activation.
More Related Videos
Related Concept Videos
01:25Immune Surveillance by NK Cells and Phagocytes
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
01:15Differentiation of Common Myeloid Progenitor Cells
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
01:28Cells of the Innate Immune Response
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...