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Articles linked to this work by shared authors, journal, and citation graph.

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Experimental autoimmune encephalomyelitis in the rat.

Current protocols in immunology·2009
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Myelin basic protein-reactive T cells persist in an inactive state in the bone marrow of Lewis rats that have recovered from autoimmune encephalomyelitis.

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Related Experiment Video

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Modeling Multiple Sclerosis in the Two Sexes: MOG35-55-Induced Experimental Autoimmune Encephalomyelitis
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Experimental autoimmune encephalomyelitis in the rat.

Robert H Swanborg1, Jolie A Stepaniak1

  • 1Wayne State University School of Medicine, Detroit, Michigan.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary

This study provides detailed protocols for inducing experimental autoimmune encephalomyelitis (EAE) in SJL mice using myelin proteins. It covers both active induction and adoptive transfer methods for EAE research.

Area of Science:

  • Neuroimmunology
  • Autoimmune disease research

Background:

  • Experimental autoimmune encephalomyelitis (EAE) is a key animal model for studying demyelinating diseases like multiple sclerosis.
  • Induction of EAE typically involves specific mouse strains and immunogenic components of myelin.

Purpose of the Study:

  • To provide standardized materials and methods for active induction and adoptive transfer of EAE in SJL mice.
  • To detail the purification processes for key myelin proteins, proteolipid protein (PLP) and myelin basic protein (MBP).

Main Methods:

  • Utilizing intact PLP and MBP proteins or peptides for EAE induction in SJL mice.
  • Describing protocols for both active immunization and adoptive transfer of T cells.
  • Outlining methods for purifying PLP and MBP.

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Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis
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Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis

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Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines
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Last Updated: Jul 5, 2026

Modeling Multiple Sclerosis in the Two Sexes: MOG35-55-Induced Experimental Autoimmune Encephalomyelitis
05:44

Modeling Multiple Sclerosis in the Two Sexes: MOG35-55-Induced Experimental Autoimmune Encephalomyelitis

Published on: October 13, 2023

Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis
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Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis

Published on: July 4, 2007

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Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines

Published on: September 21, 2021

Main Results:

  • Established protocols for reproducible EAE induction in the SJL mouse strain.
  • Successfully purified PLP and MBP for use in EAE models.

Conclusions:

  • The provided protocols facilitate consistent EAE induction in SJL mice.
  • Adaptability of these methods to other mouse strains may require protocol modification.