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Related Experiment Videos

Influenza virus.

R Cottey1, C A Rowe, B S Bender

  • 1University of Florida College of Medicine, Gainesville, Florida, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary
This summary is machine-generated.

This study details methods for mouse influenza virus infection, propagation, and titration. It also covers measuring lethal dose and generating cytotoxic T lymphocytes (CTL) for influenza research.

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Area of Science:

  • Virology
  • Immunology
  • Animal Models

Background:

  • Influenza virus infection in mice is a critical model for studying viral pathogenesis and immune responses.
  • Standardized protocols are essential for reproducible research in influenza virus infection models.

Purpose of the Study:

  • To provide a comprehensive set of methods for working with influenza virus in mouse models.
  • To enable accurate quantitation of virus and immune cell responses.
  • To facilitate the study of influenza virus adaptation and host immunity.

Main Methods:

  • Infection of mice with influenza virus.
  • Propagation and titration of influenza virus in hen eggs and cell cultures.
  • Quantitation of virus titers in serum and tissue culture medium.

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  • Measurement of the 50% mouse lethal dose (MLD50).
  • Generation and harvesting of anti-influenza cytotoxic T lymphocytes (CTL) from mouse spleens and lungs.

    • Main Results:

    • Established protocols for successful mouse infection with influenza virus.
    • Validated methods for accurate virus titration.
    • Demonstrated procedures for generating and analyzing influenza-specific CTL responses.
    • Provided a framework for adapting human influenza isolates for mouse studies.

    Conclusions:

    • The presented methods offer a robust toolkit for influenza virus research in mouse models.
    • These protocols support the investigation of viral load, pathogenicity, and cell-mediated immunity.
    • This unit facilitates standardized and reproducible studies of influenza virus infection and host response.