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Related Concept Videos

Gastritis II: Pathophysiology01:26

Gastritis II: Pathophysiology

The pathophysiology of gastritis begins with the colonization of the stomach lining by Helicobacter pylori (H. pylori). This bacterium spreads mainly via the oral-oral route through saliva or shared utensils, and can also be transmitted in overcrowded or unhygienic environments through contaminated water, despite its brief survival outside the body.ColonizationOnce ingested, H. pylori enters the stomach and begins colonization by navigating through the mucus layer lining the stomach wall. It...

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Related Experiment Video

Updated: Jun 30, 2026

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis
11:04

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis

Published on: February 19, 2013

Helicobacter animal models.

J G Nedrud1, T G Blanchard

  • 1Case Western Reserve University, Cleveland, Ohio, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary

This study details essential protocols for cultivating Helicobacter bacteria and infecting animal models, including mice and ferrets. These methods are crucial for studying Helicobacter pylori and related species in research settings.

Area of Science:

  • Microbiology
  • Animal Models
  • Immunology

Background:

  • Helicobacter infections pose significant health challenges.
  • Understanding Helicobacter pathogenesis requires robust experimental models.
  • Standardized protocols are needed for reliable research.

Purpose of the Study:

  • To provide comprehensive protocols for Helicobacter cultivation and animal infection.
  • To detail methods for quantifying bacterial load and assessing host response.
  • To establish standardized techniques for Helicobacter research.

Main Methods:

  • Cultivation of Helicobacter organisms on plates and in liquid media.
  • Infection of mice with Helicobacter felis and H. pylori, and ferrets with H. mustelae.
  • Adaptation of H. pylori isolates for murine models.

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Mouse Models Of Helicobacter Infection And Gastric Pathologies
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A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence

Published on: October 11, 2018

Related Experiment Videos

Last Updated: Jun 30, 2026

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis
11:04

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis

Published on: February 19, 2013

Mouse Models Of Helicobacter Infection And Gastric Pathologies
07:43

Mouse Models Of Helicobacter Infection And Gastric Pathologies

Published on: October 18, 2018

A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence
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  • Quantification of Helicobacter, growth curve determination, and tissue section preparation.
  • Morphological, biochemical, and rapid urease tests for identification.
  • Preparation of Helicobacter lysates and outer-membrane proteins for immune assays.

    • Main Results:

    • Established protocols for successful Helicobacter culture and animal infection.
    • Developed methods for accurate quantification and characterization of Helicobacter species.
    • Provided techniques for assessing gastric disease processes and immune responses.

    Conclusions:

    • The presented protocols offer a comprehensive toolkit for Helicobacter research.
    • These methods facilitate the study of Helicobacter pathogenesis and host-pathogen interactions.
    • Standardized procedures enhance reproducibility and comparability in Helicobacter studies.