Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced T-cell activation and differentiation in lymphocytes from transgenic mice expressing ubiquitination-resistant 2KR LAT molecules.

Gene therapy·2015
Same author

Inhibition of TGF-β signaling in genetically engineered tumor antigen-reactive T cells significantly enhances tumor treatment efficacy.

Gene therapy·2012
Same author

Induction of TLR4-dependent CD8+ T cell immunity by murine β-defensin2 fusion protein vaccines.

Vaccine·2011
Same author

Development of optimal bicistronic lentiviral vectors facilitates high-level TCR gene expression and robust tumor cell recognition.

Gene therapy·2008
Same author

Mining the melanosome for tumor vaccine targets: P.polypeptide is a novel tumor-associated antigen.

Cancer research·2001
Same author

Immunization against endogenous retroviral tumor-associated antigens.

Cancer research·2001

Related Experiment Video

Updated: Jul 5, 2026

Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs
07:31

Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs

Published on: May 24, 2016

B16 as a mouse model for human melanoma.

W W Overwijk1, N P Restifo

  • 1National Cancer Institute, NIH, Bethesda, Maryland, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary

This study outlines methods for creating B16 melanoma models in mice to test cancer immunotherapies. It details using granulocyte-macrophage colony-stimulating factor (GM-CSF) and recombinant vaccinia virus (rVVmTRP-1) for tumor protection and vitiligo induction.

Area of Science:

  • Immunology
  • Oncology
  • Virology

Background:

  • B16 melanoma is a common model for studying melanoma growth and metastasis.
  • Developing effective immunotherapies for melanoma remains a critical challenge.
  • Autoimmune responses, like vitiligo, can be associated with anti-tumor immunity.

Purpose of the Study:

  • To provide detailed protocols for establishing in vivo models of subcutaneous B16 melanoma and pulmonary metastases.
  • To describe therapeutic strategies utilizing B16.GM-CSF and rVVmTRP-1 for tumor protection and vitiligo induction.
  • To outline methods for the induction, maintenance, and adoptive transfer of gp100-specific cytotoxic T lymphocytes (CTL).

Main Methods:

  • Establishment of subcutaneous and pulmonary B16 melanoma models in mice.

More Related Videos

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine
09:15

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

Published on: February 24, 2023

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
08:19

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

Related Experiment Videos

Last Updated: Jul 5, 2026

Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs
07:31

Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs

Published on: May 24, 2016

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine
09:15

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

Published on: February 24, 2023

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
08:19

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

  • Administration of B16.GM-CSF and rVVmTRP-1 for therapeutic intervention.
  • Induction, isolation, testing, maintenance, and adoptive transfer of gp100-specific CTL.
  • Detection of MDA-specific antibodies in mouse sera using immunoblotting and ELISA.
  • Cultivation of B16 melanoma cells and enumeration of pulmonary metastases.

    • Main Results:

    • Protocols for generating and analyzing B16 melanoma metastasis models are detailed.
    • Therapeutic approaches using GM-CSF and rVVmTRP-1 demonstrate potential for tumor protection and vitiligo induction.
    • Methods for generating and utilizing antigen-specific CTL for adoptive immunotherapy are described.
    • Assays for antibody detection and methods for viral vaccination are included.

    Conclusions:

    • The provided protocols facilitate robust in vivo studies of melanoma and evaluation of novel immunotherapies.
    • The use of B16.GM-CSF and rVVmTRP-1 offers a dual approach targeting tumor growth and inducing immune responses.
    • gp100-specific CTL represent a promising strategy for adoptive immunotherapy against melanoma.
    • Comprehensive safety considerations are integrated into the experimental designs.