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Related Experiment Videos

Contact hypersensitivity.

A A Gaspari1, S I Katz

  • 1National Cancer Institute, Bethesda, Maryland, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary
This summary is machine-generated.

This study details a contact hypersensitivity assay to measure cell-mediated immunity. It uses a hapten-sensitized mouse model to quantify immune responses, highlighting Langerhans cells

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Area of Science:

  • Immunology
  • Dermatology
  • Cellular Biology

Background:

  • Contact hypersensitivity (CH) is a key in vivo assay for assessing cell-mediated immune function.
  • This reaction involves epidermal cells, exogenous haptens, and a delayed-type hypersensitive response.
  • Langerhans cells are crucial antigen-presenting cells initiating sensitization via CD4+ T lymphocytes.

Purpose of the Study:

  • To describe a standardized protocol for measuring and quantifying contact hypersensitivity in a murine model.
  • To elucidate the role of Langerhans cells and T lymphocytes in the CH reaction.
  • To establish a method for calculating the percent suppression of CH.

Main Methods:

  • Mice were shaved, and abdominal skin was exposed to a hapten for sensitization (afferent phase).

Related Experiment Videos

  • Baseline ear thickness was measured after 6 days.
  • The efferent phase involved epicutaneous treatment of the ear with the hapten solution, followed by ear thickness measurement approximately 24 hours later.

    • Main Results:

    • The change in ear thickness post-allergen treatment was quantifiable.
    • This change allowed for the calculation of the percent suppression of contact hypersensitivity.
    • The protocol effectively measures cell-mediated immune function.

    Conclusions:

    • The described protocol provides a reliable method for assessing cell-mediated immunity through contact hypersensitivity.
    • Langerhans cells play a critical role in initiating the sensitization phase of this immune response.
    • The assay is valuable for quantifying immune reactions and potential suppression.