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Updated: Jul 5, 2026

Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
Published on: September 20, 2019
Biological responses to chemokine superfamily members
Dennis D Taub1, Eric Schaffer1
1National Institute on Aging, NIH, Washington, D.C.
Abstract:
A detailed summary of all the chemokine subfamily members including alternative names, receptor-ligand pairs, receptor cellular distribution, and leukocyte responsiveness is presented here along with assays to measure the biological activities of chemokines on each of the major leukocyte subsets (i.e., microchemotaxis chambers, fluorescence-based assays, use of nitrocellulose filters and membranes coated with extracellular matrix proteins, cellular adhesion assays, mobilization of intracellular free calcium, actin polymerization, degranulation assays, and the use of chemokine receptor-specific antibodies in flow cytometric analysis and radiolabeled chemokine binding assays).
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