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Measurement of mouse and human interleukin 10
1University of Alberta, Edmonton, Alberta.
Interleukin 10 (IL-10) is a multifunctional cytokine produced by T cells and other cell types. It regulates activities of a variety of the cells of the immune system and may play a crucial role in cross-regulation of different types of T cells. Two methods of quantitating IL-10 are provided in this unit. The first is an enzyme-linked immunosorbent assay (ELISA) using a pair of monoclonal antibodies specific for mouse IL-10. This method has the advantages of high specificity and insensitivity to other cytokines present in test samples. The second method is a bioassay which has the advantages that both human and mouse IL-10 are detected and that functional protein is measured. Both methods have similar sensitivity.
Interleukin 10 (IL-10) is a multifunctional cytokine produced by T cells and other cell types. It regulates activities of a variety of the cells of the immune system and may play a crucial role in cross-regulation of different types of T cells. Two methods of quantitating IL-10 are provided in this unit. The first is an enzyme-linked immunosorbent assay (ELISA) using a pair of monoclonal antibodies specific for mouse IL-10. This method has the advantages of high specificity and insensitivity to other cytokines present in test samples. The second method is a bioassay which has the advantages that both human and mouse IL-10 are detected and that functional protein is measured. Both methods have similar sensitivity.
