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Isolation of T cells using rosetting procedures

Marjorie E Kanof1

  • 1Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.

Current Protocols in Immunology
|April 25, 2008
PubMed

This unit describes a procedure for separating T cells from other mononuclear cells by exploiting the unique ability of cells to bind to and form rosettes with sheep red blood cells (SRBC). This isolation method also allows recovery of the nonrosetting cell population (B lymphocytes, monocytes, and macrophages). Neuraminidase- and 2 -aminoethylisothiouronium bromide (AET)-treated SRBC are used for rosetting because of enhanced binding to T cells.

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