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Purification of T cell subpopulations

M E Kanof1

  • 1Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Current Protocols in Immunology
|April 25, 2008
PubMed

This unit describes a procedure for isolating T cell populations or subpopulations using the method of indirect panning. In this method, cells are selected by their capacity to bind to antibody-coated plates on the basis of particular cell-surface markers. It is superior to the antibody/complement lysis method (also presented) because the nonselected cell population can be retrieved.

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