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Induction of TNBS colitis in mice
Frank Scheiffele1, Ivan J Fuss
1National Institute of Allergy and Infectious Disease, NIH, Bethesda, Maryland, USA.
Current Protocols in Immunology
|April 25, 2008
Summary
This study details a mouse model for inflammatory bowel disease using 2,4,6-trinitrobenzenesulfonic acid (TNBS) to induce colitis. The model mimics Crohn's disease, offering insights into immunopathogenesis and disease monitoring.
Area of Science:
- Immunology
- Gastroenterology
- Animal Models
Background:
- Inflammatory bowel disease (IBD) pathogenesis is complex.
- Animal models are crucial for studying IBD.
- Existing models have limitations in mimicking human Crohn's disease.
Purpose of the Study:
- To describe a reliable animal model for studying inflammatory bowel disease (IBD).
- To detail the induction and assessment of colitis in mice using 2,4,6-trinitrobenzenesulfonic acid (TNBS).
- To provide methods for monitoring and grading disease severity.
Main Methods:
- Intrarectal administration of 2,4,6-trinitrobenzenesulfonic acid (TNBS) in SJL/J mice.
- Induction of severe colonic inflammation.
- Monitoring and grading of clinical and histopathological disease parameters.
- Isolation of lamina propria mononuclear cells.
Main Results:
- TNBS administration reliably induces colitis in SJL/J mice.
- The induced colitis exhibits clinical and histopathological features similar to Crohn's disease.
- Established parameters allow for successful induction and assessment of the disease model.
Conclusions:
- The TNBS-induced colitis model in SJL/J mice is a valuable tool for IBD research.
- This model facilitates the study of inflammatory bowel disease immunopathogenesis.
- The described methods support reproducible research in this area.
