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Isolation of Ly-1+/CD5+ B cells by cell sorting.
1Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA.
Current Protocols in Immunology
|April 25, 2008
Summary
This study presents a flow cytometry method to isolate CD5+ B cells, a distinct immune cell population. This technique enables the purification of specific B cell subsets for further research in immunology.
Area of Science:
- Immunology
- Cell Biology
- Flow Cytometry
Background:
- CD5 (Ly-1) is a glycoprotein initially identified on T cells, later found on B cells.
- CD5 expression marks a functionally distinct B cell subset, crucial for immunological studies.
- Isolating pure CD5+ B cells and control populations is vital for research in murine and human immune systems.
Purpose of the Study:
- To present a flow cytometry technique for isolating pure populations of CD5+ B cells.
- To enable the selection of functionally distinct B cell subsets for immunological research.
- To provide a method for obtaining both CD5+ B cells and appropriate control populations.
Main Methods:
- Utilized flow cytometry for cell sorting.
- Separated cell populations based on physical properties like size and granularity.
- Correlated cell surface molecule expression for precise isolation.
Main Results:
- Successfully selected and isolated two distinct cell populations from a complex mixture.
- The technique effectively distinguished cells based on physical characteristics and surface marker expression.
- Enabled the isolation of CD5+ B cells and relevant control groups.
Conclusions:
- The described flow cytometry method is effective for isolating pure CD5+ B cell populations.
- This technique facilitates the study of functionally distinct B cell subsets.
- Provides researchers with a reliable tool for immunological investigations involving CD5+ B cells.
