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Updated: Jul 5, 2026
08:05
Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
Analyzing mitogen-activated protein kinase (MAPK) activities in T cells
Alan J Whitmarsh1, Roger J Davis
1University of Manchester, School of Biological Sciences, Manchester, United Kingdom.
Current Protocols in Immunology
|April 25, 2008
Summary
This study details methods for measuring T cell signaling pathways, specifically focusing on JNK and ERK kinase activities. These assays are crucial for understanding T cell activation and immune responses.
Area of Science:
- Immunology
- Molecular Biology
- Cell Signaling
Background:
- T cell activation involves T cell receptors (TCR) and costimulatory receptors.
- Signaling pathways, including MAPK (ERK and JNK), are critical for T cell responses like IL-2 production.
Purpose of the Study:
- To provide detailed protocols for quantifying JNK and ERK kinase activities in stimulated T cells.
- To enable researchers to study T cell activation signaling.
Main Methods:
- Solid-phase kinase assay for JNK activity measurement.
- Immune-complex kinase assay for ERK activity measurement.
- Preparation of GSThyphen;cJun/GSH-Sepharose beads for JNK assays.
Main Results:
- Established procedures for measuring JNK and ERK kinase activities in T cells.
- Detailed methods for assay component preparation.
Conclusions:
- The presented assays are valuable tools for investigating T cell signaling pathways.
- Accurate measurement of JNK and ERK activity is essential for understanding T cell function.
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