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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
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Published on: October 28, 2014

Measurement of intracellular ions by flow cytometry

Carl H June1, Jonni S Moore1

  • 1University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania.

Current Protocols in Immunology
|April 25, 2008
PubMed

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...