Related Experiment Video
Updated: Jul 5, 2026

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus
Published on: August 25, 2017
Herpesvirus saimiri transformation of human T lymphocytes
Bernhard Fleckenstein1, Armin Ensser1
1Institut für Klinische und Molekulare Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Abstract:
Viral transformation of T cells is an effective method for obtaining large numbers of T cells that are easily maintained in the laboratory. This unit describes a method for generating antigen-independent, virally-transformed T cells using a T-lymphotropic primate gamma-2 herpesvirus, Herpesvirus saimiri (HVS; strain C488). Support protocols for preparing and titrating HSV C488 stocks and testing the functional status of transformed T cells are also included.
Related Concept Videos
Herpes
Cytomegalovirus Disease

